What do you mean by scale up in bioreactor?
Scale-up is the process of expanding a fermentation process from a smaller-scale fermenter, where operational and production parameters have been studied, to a larger scale. Scale-up is perhaps one of the hardest and most complex steps of any fermentation process for engineers.
What is the criteria for scale up?
Parameters to rely on—conventional scale-up criteria Engineering parameters such as the vessel and impeller geometry, tip speed, mixing time, oxygen transfer rate, volumetric mass transfer coefficient (kLa), or power number can be used as scale-up criteria .
What is scale up in bioprocess?
Scale-up is the process of transferring a process from small volume bench scale reactors to larger vessels for material generation for product testing or commercial production.
What are the various factors which were followed while scaling up process in the bioreactor?
- Power-to-volume ratios.
- Height-to-diameter ratios.
- Equipment geometry across scale (ratio of impeller diameter to vessel diameter, impeller blade length, width, and angle)
- Fluid dynamics (type of agitation, number of impellers, tip speed)
What is scale down in fermentation process?
Fermentation processes often involve several scales of operation, encompassing inoculum development, seed expansion, and production fermentation. The differences in volumes between the steps in a single fermentation process can be 10X to 100X for the pilot scale, and 1,000 to 100,000X for the production scale.
What is Vvm in bioreactor?
*Vessel volumes per minute (vvm) = air flow rate/operating volume. Page 6. 6. Cell Culture. Maintaining a homogenous environment within the bioreactor is the most critical criterion for a successful cell culture process.
What do you mean by scale up?
to increase something in size, amount, or production: This research on seaweed could be scaled up at low cost.
What is the purpose of scale up?
The goal of scale-up is to identify & develop a process that will successfully produce a desired product when manufactured at a commercial scale. To successfully move from the small scale to the large, one must understand how size changes impact a number of physical & chemical phenomena.
What is the importance of scale up?
Identifying, verifying and making available the current scale-up status of a business is one of the most important tools for boosting productivity, so access to better, faster data about scale-ups is crucial.
What is fermentation scale up?
The essence of scale-up of a fermentation process is to demonstrate fermentation production at large scale resulting in the same productivity and quality as that developed at small scale.
What is importance of scale up?
What is scale up manufacturing process?
To scale-up the manufacturing process, one of the first things to consider is how large a batch size you will need. The answer to that question is typically driven by your marketing plans. Your marketing plans will help you determine the optimal batch size for your product.
What do you mean by scale-up?
What is fermentation scale-up?
What is Vvm and LPM?
As VVM stands for volume of air sparged (in aerobic cultures) per unit volume of growth medium per minute, it is calculated by dividing the measured airflow rate (units: L/m, using a rotameter) by the volume (L) of growth medium (including cultured cells).
What is aeration rate?
Fermentation Design Laboratory scale work frequently reports aeration rates as the volume of air at standard conditions per volume of liquid per minute, or standard cubic feet of air per hour per gallon.
What is the meaning of scale up and scale down?
If you scale up something, you make it greater in size, amount, or extent than it used to be.
What is scale-up production?
What does it mean to scale up in manufacturing? Growing to scale (or scaling up), is when a business ensures all aspects of its processes grow in tandem so that one area isn’t left behind.
What is an example of scaling up?
Scale-up definition To scale up is defined as to make something grander or larger. An example of scale up is when you redraw a 2000 square foot house plan into a 5000 square foot house plan.
What is bioreactor scale-up?
Bioreactor scale-up is a critical step in the production of therapeutic proteins such as monoclonal antibodies (MAbs). With the scale-up criterion such as similar power input per volume or O 2 volumetric mass transfer coefficient ( kLa), adequate oxygen supply and cell growth can be largely achieved.
How to scale up bioreactor with pseudohomogeneous bioprocesses?
The most often used criteria for the scale-up of bioreactor with pseudohomogeneous bioprocesses are: geometrical similarity, mixing or circulation time, residence or circulation time distribution, specific power input, Reynolds rotation number or stirrer tip speed.
What are the disadvantages of scale up in bioreactors?
impeller diameter of scale- up •High shear rates can cause the cell membrane to tear and the cells to die. •If scale-up based on constant tip speed is attempted, P/V and mixing time will decrease Bioreactor Scaling up Scaling up / Scaling Down
What are the key parameters of a bioreactor?
The success of scale-up depends on sound engineering and scientific characterization of the bioreactor system, including engineering parameters such as kLa, P/V, mixing times and shear stress distribution. Learn more about Controlling the Key Parameters of a Bioreactor.