What is RNase DNase?

What is RNase DNase?

DNase I (RNase-free) is a recombinant endonuclease that nonspecifically catalyzes the degradation of both single- and double-stranded DNA and DNA-RNA hybrids, producing 5′-phosphate and 3′-hydroxyl termini-containing oligonucleotides of varying lengths.

What does DNase enzyme do?

Deoxyribonuclease (DNase) enzymes perform a variety of important cellular roles by degrading DNA via hydrolysis of its phosphodiester backbone. Deoxyribonuclease I (DNase I) enzymes cleave single or double-stranded DNA and require divalent metal ions to hydrolyze DNA yielding 3΄-hydroxyl and 5΄-phosphorylated products.

Is Ambion an Invitrogen?

Ambion is now Invitrogen. Backed by 30 years of experience, our scientists and technical support teams are knowledge leaders in RNA research, and experts in RNA purification. Invitrogen technical resources—including technical notes, protocols, fundamentals, and more—demonstrate our wealth of expertise.

What does DNase do in PCR?

A frequent use of DNase I is to treat RNA preparations to degrade trace to moderate amounts of genomic DNA (up to 10 µg/ml) that could otherwise result in false positive signals in subsequent RT-PCR. The amount of RNA that can be treated in a single DNase I reaction will depend on the amount of DNA contamination.

What is RNase free DNase set?

The RNase-Free DNase Set provides efficient on-column digestion of DNA during RNA purification from cells and tissues using RNeasy Kits and the QIAamp RNA Blood Mini Kit. The DNase is efficiently removed in subsequent wash steps.

Does DNase affect RNA?

High temperatures necessary for the inactivation of the DNase I enzyme can also lead to RNA degradation. The heat inactivation of the DNase I enzyme requires an incubation at +75°C for 15 minutes. Since RNA molecules are heat sensitive (Huang et al., 1996), this incubation time should be as short as possible.

How does DNase digest DNA?

Deoxyribonuclease I (DNase I) is an endonuclease which is secreted to cleave DNA in the extracellular space down to an average of tetranucleotides with 5′ monophosphate and 3′ hydroxyl DNA ends (Baranovskii, Buneva, & Nevinsky, 2004). Both single-stranded DNA and double-stranded DNA are degraded by DNase I.

Where are DNases found?

DNase I is produced mainly by organs of the digestive system, such as the pancreas and salivary parotid glands. Therefore, three types of mammalian DNase I are known: pancreatic, parotid and pancreatic-parotid [10].

Is Thermofisher and Invitrogen same?

Life Technologies was acquired by Thermo Fisher Scientific in January, 2014. Our innovative life science, applied science, and clinical products are now found under the Applied Biosystems™, Invitrogen™, Gibco™, Ion Torrent™, and Molecular Probes™ brands.

Is Life Technologies Invitrogen?

In 2000, Invitrogen acquired Life Technologies and discontinued that name.

Why is DNase used in RNA extraction?

For removal of genomic DNA from RNA samples, a DNase I treatment is recommended. The DNAse I enzyme is classified as an endonuclease which is able to digest single and double-stranded DNA into single bases or oligonucleotides.

Why is DNase a virulence factor?

DNases have often been described as virulence factors in streptococci [31] or staphylococci [14]. Indeed, it has been shown that DNase can help bacteria to escape from neutrophil extracellular traps (NETs) which are structures secreted by neutrophils to trap and kill bacteria [32].

Can I Vortex DNase?

For more information, consult the appropriate safety data sheets (SDSs), available from the product supplier. Do not vortex the DNase I. It will denature the enzyme and decrease its activity.

Can DNase digest RNA?

How does DNase treat RNA?

Heat inactivation: Probably the most common method of DNase inactivation is heat treatment, typically for 5 minutes at 75°C. Although this method appears straightforward, the divalent cations in the DNase digestion buffer can cause (chemically-induced) strand scission of RNA when heated.

Does DNase exist in humans?

“DNase I is present in the chief cells of human and rat stomachs”.

What produces DNase?

In order to utilize external DNA, bacteria cells secrete exoenzymes (DNases) outside of the cell that hydrolyze DNA into nucleotides. The nucleotides can then move across the cell membrane via transport proteins to be utilized.

Is Fisher and Thermofisher the same company?

In May 2006, Fisher Scientific and Thermo Electron announced that they would merge in a tax-free, stock-for-stock exchange. The merger closed on November 9, 2006 and the merged company is now called Thermo Fisher Scientific. The merger was valued at $12.8 billion.

Who owns Thermo Fisher Scientific?

Thermo Fisher ScientificThermo Fisher Scientific / Parent organization

How does DNase treat RNA sample?

Tip: As a rule of thumb for the DNase I digestion, use one unit of DNase I per 1 to 5 μg of total RNA in a 50 μl total volume incubated for 20 minutes at +25 to +37°C. After the additional DNase digestion step an additional purification of the RNA from the DNase I enzyme is mandatory.

What is Ambion DNase I (RNase free)?

Ambion DNase I (RNase-free) (E.C. is a nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin.

What is an RNase-free DNase I?

RNase-free DNase I is of the highest purity available and is recommended to degrade DNA in the presence of RNA when the absence of RNase is critical to maintain the integrity of the RNA.

What is the pH of DNase I?

DNase I requires bivalent cations (Mg 2+ and Ca 2+ at approximately 5 mM and 0.5 mM, respectively) for maximal activity, and has a pH optimum of 7.8. A research report in BioTechniques (Matthews et al., 32: 1412-1417, 2002) compared RNase contamination in DNase I preparations from Sigma, Roche, Applied Science, Qiagen, and Ambion.

What is the function of DNase 1?

It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5′-phosphate and a 3′-hydroxyl group. RNase-free DNase I is of the highest purity available and is recommended to degrade DNA in the presence of RNA when the absence of RNase is critical to maintain the integrity of the RNA.