What is a polymethine chain?

What is a polymethine chain?

: any of a class of dyes (as cyanines or merocyanines) characterized by a resonance structure containing a conjugated chain of carbon atoms that is at least in part an open chain [as in −CH(=CH−CH)n=] attached to two polar atoms (as two amino nitrogen atoms either one of which is considered to be positively charged)

What are cyanine dyes used for?

Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques (Ünlü et al., 1997; Osterman et al., 2013) [5,6].

What Colour is Cy5?

Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiD and its variants.

How big is Cy3?

Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).

Is Cy3 red?

Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

Is Cy5 far red?

Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines.

ARE FAM and FITC the same?

FITC and FAM are both derivatives of fluorescein. FITC is fluorescein with an isothiocyanate reactive group added, while 6-FAM is 6-carboxyfluorescein. FITC and 6-FAM have identical spectral characteristics. In experiments, 6-FAM performs exactly like FITC.

Is FITC same as 488?

Fluorescent conjugates The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.

Does FITC bleed into PE?

Why do we need compensation? where FITC bleeds into the PE channel and PE bleeds back into FITC. To correct for spectral overlap during multicolor flow cytometry experiments, color compensation must be performed. The goal of color compensation is to correctly quantify each dye with which a particular cell is labeled.

Is FITC same as GFP?

Available Options. Green Fluorescent Protein (GFP) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC against GFP.

Is Alexa Fluor 488 the same as FITC?

Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. However, Alexa Flour 488 display higher photostability and is less susceptible to self-quenching.

Can you use PE and FITC together?

FITC overlap a lot with Pe, don’t use them together on same cells. PerCP-‐Cy5. 5 overlap surprisingly a lot with BV711, don’t use together.

Can I use PE and FITC?

In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. In those cases the relative contribution of each fluorophore to the signal in a given detector must be determined (Figure 11).