What are BSL-2 safety precautions?
What are BSL-2 safety precautions?
BSL-2, continued
- Appropriate personal protective equipment (PPE) is worn, including lab coats and gloves.
- All procedures that can cause infection from aerosols or splashes are performed within a biological safety cabinet (BSC) .
- An autoclave or an alternative method of decontamination is available for proper disposals.
What is the difference between BSL2 and BSL2 +?
Biosafety Level 2+ (BSL2+) This biosafety level is also referred to as “Biosafety Level 2 Enhanced”. Work conducted at BSL2+ usually involves work with biological agents that would normally be conducted at BSL2; however, the work may involve certain conditions which would necessitate an increased amount of precautions.
Which of the following are requirements for a BSL2 laboratory?
In a BSL-2 lab, the following conditions are to exist:
- Doors. Doors that can be locked and secured should be installed for facilities that house restricted areas.
- Public.
- Sink.
- Cleaning.
- Bench Tops.
- Lab Furniture.
- Biological Safety Cabinets.
- Eyewash Station.
What constitutes BSL2?
BSL–2. This biosafety level covers laboratories that work with agents associated with human diseases (i.e. pathogenic or infections organisms) that pose a moderate health hazard.
What is biosafety laboratory 2 or bsl2?
BSL-2 laboratories are used to study moderate-risk infectious agents or toxins that pose a moderate danger if accidentally inhaled, swallowed, or exposed to the skin. Design requirements for BSL-2 laboratories include hand washing sinks, eye washing stations, and doors that close and lock automatically.
What is the difference between BSL 1 and BSL-2?
The main difference in the work procedures followed in a BSL-1 laboratory and a BSL-2 laboratory is that employees in a BSL-2 laboratory will use a BSC as a primary barrier for potentially hazardous aerosols.
What is the difference between bsl1 and bsl2?
Which biosafety cabinet is usually required when working with biological materials?
Biosafety Level 1 applies when working with biological agents, which pose a minimal risk/ threat to laboratory personnel and the environment. Work with these types of agents are generally performed in open laboratory cabinets without the use of special containment equipment.
What are the guidelines for laboratory facilities and safety equipment?
General Laboratory Safety Rules
- Know locations of laboratory safety showers, eyewashstations, and fire extinguishers.
- Know emergency exit routes.
- Avoid skin and eye contact with all chemicals.
- Minimize all chemical exposures.
- No horseplay will be tolerated.
- Assume that all chemicals of unknown toxicity are highly toxic.
What are the three primary routes of transmission at BSL-2?
inhalation, 2. direct contact with skin or mucous membranes, 3. ingestion, and 4.
What does BSL 3 mean?
Biosafety Level 3
“BSL3” stands for Biosafety Level 3 and biocontainment facilities. According to CDC and WHO guidelines, “Biosafety Level 3 is applicable to laboratories where work with indigenous or exotic agents may cause serious disease because of exposure by inhalation route.
Are human samples bsl2?
Some body fluids such as sweat and urine are not generally known to contain agents and not considered biohazardous. The processing or testing of biohazardous human specimens should be done at Biosafety Level 2 (BSL-2).
Is BSL-2 a blood?
BSL-2 is the biosafety level used for work with human blood, body fluids, or tissues where the presence of an infectious agent may be unknown.
How should you handle a small spill in the biosafety cabinet?
Lesser spills, even the smallest amount, should be immediately treated with the appropriate disinfectant for your lab. After sufficient contact time, wipe up with paper towels. Surfaces treated with bleach should be rinsed immediately with sterile water to avoid damage to the surface metal of the cabinet.
What are the 3 biological safety cabinets?
There are three classes of biosafety cabinets designated in the United States: Class I, Class II, and Class III. Class I biosafety cabinets are infrequently used and provide personnel and environmental protection but no product protection.
What are 5 safety precautions for a laboratory?
Basic Safety Rules
- Know locations of laboratory safety showers, eyewashstations, and fire extinguishers.
- Know emergency exit routes.
- Avoid skin and eye contact with all chemicals.
- Minimize all chemical exposures.
- No horseplay will be tolerated.
- Assume that all chemicals of unknown toxicity are highly toxic.
What are the best practices for safely handling waste in the lab?
What are the best practices for safely handling waste in the lab? Always wear appropriate Personal Protective Equipment. Remove gloves for better manipulation of the waste containers. Place any paper towels from the clean-up in the trash.
What is a BSL-4?
Biosafety Level 4 (BSL-4) BSL-4 laboratories are used to study infectious agents or toxins that pose a high risk of aerosol-transmitted laboratory infections and life-threatening disease for which no vaccines or therapies are available.
How do you deal with biological spills?
Cover the spill area with absorbent materials such as Green-Z, paper towels, SSS clean-up powder. Remove the absorbent material. If using a powder or solidifier, use dustpan and broom. Dispose of all absorbent materials and tools into a biohazard bag.
How will you handle and clean spills of infectious material in the laboratory?
Contain the spill with absorbent paper towels or disposable pads. Carefully add appropriate disinfectant to the spill; avoid creating aerosols when pouring the disinfectant. Leave the room and allow 30 minutes for the disinfectant to inactivate the material.
What to do if you spill BSL 2?
BSL-2 Spill Avoid inhaling airborne material while quickly leaving the room. Notify others to leave. Close the door and post with a warning sign. Remove contaminated clothing, turn exposed areas inward and place in a biohazard bag. Wash all exposed skin with disinfectant.
What are the risks of bsl2?
Primary concerns at BSL2 include accidental needlesticks or cuts or mucous membrane exposures, or ingestion of infectious materials. Even though organisms routinely manipulated at BSL2 are not known to be transmissible by the aerosol route, procedures with aerosol or high splash potential may increase the risk of personnel exposure.
What is a BSL 2 experiment?
Biosafety Level 2 (BSL-2) is suitable for experiments involving agents of moderate potential hazard to personnel and the environment. For example: Microorganisms of low biohazard potential, such as those in Risk Group 2 or BSL-2.
Is it safe to use aerosol at bsl2?
Even though organisms routinely manipulated at BSL2 are not known to be transmissible by the aerosol route, procedures with aerosol or high splash potential may increase the risk of personnel exposure. Limit access to work areas. Close doors during work with research materials.